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Journal Article 9

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pig 3

PRRSV 2

embryonic stem cells 2

induced pluripotent stem cells 2

Drosophila S2 cell 1

Nsp9 1

DNase I 1

GFP 1

ICSI 1

OTX2 1

PEDV 1

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V-set Ig-like domain 1

antibody 1

antiviral 1

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Structural prediction of porcine sialoadhesin V-set Ig-like domain sheds some light on its role in porcine

Jie HOU,Rui LI,Hongfang MA,Songlin QIAO,Gaiping ZHANG

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1,   Pages 65-71 doi: 10.15302/J-FASE-2016086

Abstract: Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failures in sowsPorcine sialoadhesin (pSn) is a putative receptor of PRRSV.

Keywords： PRRSV     porcine sialoadhesin     V-set Ig-like domain     Drosophila S2 cell     crystallization    

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Effect of calcium on porcine ICSI embryos expressing EGFP is related to activation of ooplasmic DNase

Shuaishuai WU,Heng CHEN,Yingzheng WANG,Hui GAO,Shenming ZENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 1,   Pages 84-89 doi: 10.15302/J-FASE-2015046

Abstract: To date, the effect of different calcium concentrations in manipulation media on porcine intracytoplasmic

Keywords： ICSI     calcium     DNase I     GFP     porcine    

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Progress, problems and prospects of porcine pluripotent stem cells

Hanning WANG,Yangli PEI,Ning LI,Jianyong HAN

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1,   Pages 6-15 doi: 10.15302/J-FASE-2014009

Abstract: Efforts to derive porcine ESCs and iPSCs have not yielded high-quality PSCs that can produce chimerasThus, exploration of the unique porcine gene regulation network of preimplantation embryonic developmentmay permit optimization of culture systems for raising porcine PSCs.Here we summarize the recent progress in porcine PSC generation as well as the problems encountered duringthis progress and we depict prospects for generating porcine naive PSCs.

Keywords： induced pluripotent stem cells     embryonic stem cells     pig     reprogramming    

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Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 66-72 doi: 10.15302/J-FASE-2018229

Abstract:

Porcine viral diarrhea is an acute and highly contagious enteric disease of pigs that causes hugePorcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are the main pathogensThese vectors were then transfected into porcine fetal fibroblasts, G418 resistant transfectants wereconfirmed by PCR and transgenic SCNT porcine blastocysts were obtained.

Keywords： piglet diarrhea     RNAi     PEDV     TGEV     transgenic resistance breeding    

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SALL4 maintains self-renewal of porcine pluripotent stem cells through downregulation of OTX2

Ning WANG, Sile WANG, Yaxian WANG, Yuanxing CAI, Fan YANG, Huayan WANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 81-92 doi: 10.15302/J-FASE-2017180

Abstract: However, the molecular features and function of SALL4 have not been well elucidated in porcine inducedIn this study, we identified splice variants and found two splicing variants through analysis of the porcineSALL4A was only detected in piPSCs but SALL4B was globally expressed in porcine tissues and piPSCs.

Keywords： OTX2     pluripotency     pig     SALL4     transcription regulation    

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Characterization of polyclonal antibodies against nonstructural protein 9 from the porcine reproductive

Mengmeng ZHAO,Juanjuan QIAN,Jiexiong XIE,Tiantian CUI,Songling FENG,Guoqiang WANG,Ruining WANG,Guihong ZHANG

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 2,   Pages 153-160 doi: 10.15302/J-FASE-2016097

Abstract: Porcine reproductive and respiratory syndrome (PRRS) is considered to be one of the most important infectious

Keywords： PRRSV     Nsp9     expression     antibody     neutralize    

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Transcriptomic basis of neutrophil ratio variation induced by poly I:C stimulation in porcine peripheral

Haiyan WANG, Qiaoxia ZHANG, Lilin YIN, Xiangdong LIU, Shuhong ZHAO, Mengjin ZHU, Changchun LI

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 3,   Pages 342-352 doi: 10.15302/J-FASE-2017162

Abstract: Using Affymetrix porcine genome microarrays, we investigated the gene expression profiles associatedwith neutrophil variation in porcine peripheral blood before and after polyriboinosinic-polyribocytidylic

Keywords： neutrophil     peripheral blood     pig     poly I:C     transcriptome    

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A brief review of microRNA and its role in PRRSV infection and replication

Xuekun GUO,Wenhai FENG

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 2,   Pages 114-120 doi: 10.15302/J-FASE-2014022

Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV), a single-stranded RNA virus, mainly infects

Keywords： porcine reproductive and respiratory syndrome virus (PRRSV)     microRNA     antiviral     viral tropism    

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Porcine pluripotent stem cells: progress, challenges and prospects

Jianyong HAN, Yi-Liang MIAO, Jinlian HUA, Yan LI, Xue ZHANG, Jilong ZHOU, Na LI, Ying ZHANG, Jinying ZHANG, Zhonghua LIU

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 8-27 doi: 10.15302/J-FASE-2018233

Abstract: However, lack of knowledge about the derivation, characterization and pluripotency mechanisms of porcineIn this review, we discuss the latest progress on porcine PSCs generation, evaluation criteria for pluripotencyWe also introduce our perspectives on porcine PSC research, in the hope of providing new ideas for generatingnaive porcine PSCs and animal breeding.

Keywords： embryonic germ cells     embryonic stem cells     induced pluripotent stem cells     pigs     pluripotent stem cells    

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Title Author Date Type Operation

Structural prediction of porcine sialoadhesin V-set Ig-like domain sheds some light on its role in porcine

Jie HOU,Rui LI,Hongfang MA,Songlin QIAO,Gaiping ZHANG

Journal Article

Effect of calcium on porcine ICSI embryos expressing EGFP is related to activation of ooplasmic DNase

Shuaishuai WU,Heng CHEN,Yingzheng WANG,Hui GAO,Shenming ZENG

Journal Article

Progress, problems and prospects of porcine pluripotent stem cells

Hanning WANG,Yangli PEI,Ning LI,Jianyong HAN

Journal Article

Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Journal Article

SALL4 maintains self-renewal of porcine pluripotent stem cells through downregulation of OTX2

Ning WANG, Sile WANG, Yaxian WANG, Yuanxing CAI, Fan YANG, Huayan WANG

Journal Article

Characterization of polyclonal antibodies against nonstructural protein 9 from the porcine reproductive

Mengmeng ZHAO,Juanjuan QIAN,Jiexiong XIE,Tiantian CUI,Songling FENG,Guoqiang WANG,Ruining WANG,Guihong ZHANG

Journal Article

Transcriptomic basis of neutrophil ratio variation induced by poly I:C stimulation in porcine peripheral

Haiyan WANG, Qiaoxia ZHANG, Lilin YIN, Xiangdong LIU, Shuhong ZHAO, Mengjin ZHU, Changchun LI

Journal Article

A brief review of microRNA and its role in PRRSV infection and replication

Xuekun GUO,Wenhai FENG

Journal Article

Porcine pluripotent stem cells: progress, challenges and prospects

Jianyong HAN, Yi-Liang MIAO, Jinlian HUA, Yan LI, Xue ZHANG, Jilong ZHOU, Na LI, Ying ZHANG, Jinying ZHANG, Zhonghua LIU

Journal Article